Journal: The Journal of Physiology

Article Title: Bile acids induce necrosis in pancreatic stellate cells dependent on calcium entry and sodium‐driven bile uptake

doi: 10.1113/JP272774

Figure Lengend Snippet: A , mRNA expression of selected genes encoding bile acid transporters in human pancreatic stellate cells (hPSCs) and human hepatocytes. PCR products for the sodium–taurocholate cotransporting polypeptide (NTCP, slc10A1) and sodium independent transporters, OATP4A1 ( slco4A1 ) and OATP1B3 (slco1B3) , are present in human hepatocytes; hPSCs express slc10A1 and slco4A1 , but not slco1B3 . B , immunoblotting shows expression of NTCP in different tissues; lines from the left: mass marker, hPSC, four positive controls (human liver and mouse pancreas, liver and kidney), two negative controls (mouse spleen and lung). C , immunohistofluorescence (IHF) staining for NTCP in fixed mouse tissue sections: the pancreas (upper panel) and the liver (lower panel). From the left: DAPI (blue), NTCP (white), overlay images (white arrows indicate PSCs), and transmitted light images. Insets show corresponding staining in controls without primary antibody. D , double IHF staining for NTCP and the bradykinin receptor B2 (BDKRB2). From the upper left: DAPI (blue), NTCP (green), BDKRB2 (red), fluorescence overlay (PSCs are indicated by white arrows). E and F , the comparison of homological sequences of three Na + ‐dependent mouse transporters (NTCP, ASBT and SOAT) with immunogens used for generation of the antibodies for immunoblotting ( E ) and IHF ( F ). Red letters represent amino acids identical to those in the immunogen sequence; blue letters are similar amino acids, and black letters show amino acids that are different. Only mouse NTCP sequences share high sequence identity with the immunogens.

Article Snippet: Human liver tissue lysate was obtained from Novus Biologicals, Littleton, CO, USA.

Techniques: Expressing, Western Blot, Marker, Immunohistofluorescence, Staining, Fluorescence, Comparison, Sequencing

Journal: Oncotarget

Article Title: KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes

doi:

Figure Lengend Snippet: ( A and B ) Flow cytometric analysis of surface KIAA1114 expression in human CC cell lines (A) and AFP + and AFP − HCC cell lines (B) . (C) Immunoblot analysis of tissue lysates from normal liver (N), patient tumor (PT), and its adjacent normal tissue from the same donor (PN). β-actin served as an internal control.

Article Snippet: Human liver whole tissue lysate and paired primary tumor and normal tissues were purchased from Novus Biologicals.

Techniques: Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Subfamily-selective PCR primers for the human LINE1 L1PA lineage

doi: 10.1038/s41598-025-17649-z

Figure Lengend Snippet: Quantification of L1PA subfamily RNA signals in human liver using subfamily-selective PCR primers. ( a ) LINE1 RT-qPCR analysis of human liver RNA using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 technical replicates. ( b ) RNA-seq analysis of L1PA subfamilies in human liver using publicly available data. Summed FPKM values of annotated loci per L1PA subfamily are shown. Data are presented as mean ± s.d., n = 3 biological replicates. Data include multi-mapping reads; a corresponding analysis restricted to unique alignments is presented in Supplementary Fig. 3. ( c ) LINE1 RT-qPCR analysis of HEK293T cells following treatment with 5-azacytidine using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 biological replicates. Statistical significance was assessed using a two-tailed t-test and *, ** and *** indicate p < 0.05, 0.01 and 0.001, respectively.

Article Snippet: cDNA synthesis was performed on human liver RNA (Amsbio R1234151-50) with SuperScript II Reverse Transcriptase (ThermoFisher).

Techniques: Quantitative RT-PCR, Standard Deviation, RNA Sequencing, Two Tailed Test